Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
AR

Cell type

Cell type Class
Breast
Cell type
ZR-75-1
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
ZR-75-1_E2+DHT
cell line
ZR-75-1
cell type
Breast cancer cell line
passages
14-17 (where replicates represent sequential passages)
treatment
E2+DHT
chip antibody
AR (N-20) (Santa Cruz SC-816)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ZR-75-1 cells were fixed with formaldehyde for 10m prior to quenching. Chromatin was prepared and subsequently sheared by the Diagenode BioRuptor Plus for 10 cycles (30 sec on, 30 sec off) on ice. Sheared chromatin was immunoprecipitated with 10µg of AR or ERα antibody, or 2µg H3K27Ac antibody overnight, after coupling to Protein A Dynabeads (10002D). DNA was purified using phenol:chloroform:isoamyl alcohol extraction. Libraries were prepared according to manufacturer's instructions accompanying the Qiagen Ultralow Input Library Kit (Part# 180495). ChIP-Seq; Illumina NextSeq500 (High Output v2) single-end 75 bp reads.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
63099608
Reads aligned (%)
98.3
Duplicates removed (%)
5.3
Number of peaks
21823 (qval < 1E-05)

hg19

Number of total reads
63099608
Reads aligned (%)
97.6
Duplicates removed (%)
6.7
Number of peaks
21456 (qval < 1E-05)

Base call quality data from DBCLS SRA